Protein Analysis of Skimmed Milk Powder by Titration Method using Sodium Hydroxide and Hydrochloric Acid:
Materials:
- Skimmed milk powder
- Sodium hydroxide (NaOH)
- Hydrochloric acid (HCl)
- Burette
- Pipette
- Funnel
- Conical flask
- Glass rod
- 0.01 N NaOH solution
- 0.01 N HCl solution
- Phenolphthalein indicator
Standard Solution Preparation:
- Take 0.01 N NaOH solution in a burette.
- Rinse the burette several times with distilled water.
- Fill the burette with the NaOH solution up to the zero mark.
Standardization of Sodium Hydroxide Solution:
- Take 25 mL of 0.01 N HCl solution in a conical flask.
- Add a few drops of phenolphthalein indicator to the solution.
- Record the initial burette reading (V1).
- Slowly titrate the NaOH solution into the HCl solution while swirling the flask until the solution turns a faint pink color.
- Record the final burette reading (V2).
- Repeat the titration until two consecutive titrations give the same volume of NaOH solution.
- Calculate the molarity of the NaOH solution using the following formula:M = N * (V2-V1) / 25
Test Method for Protein Analysis:
- Take 1 g of skimmed milk powder in a conical flask.
- Add 50 mL of distilled water to the flask.
- Mix the solution thoroughly using a glass rod.
- Add 2 drops of phenolphthalein indicator to the solution.
- Record the initial burette reading (V1).
- Slowly titrate the NaOH solution into the solution while swirling the flask until the solution turns a faint pink color.
- Record the final burette reading (V2).
- Calculate the amount of NaOH solution required to neutralize the sample using the following formula:V = (V2 - V1)
- Calculate the protein content in the sample using the following formula:Protein (g) = (V * M * 16) / 1000
Note: The factor 16 is used to convert the amount of NaOH from moles to milligrams.
The above method can be used to determine the protein content in skimmed milk powder using titration with sodium hydroxide and hydrochloric acid. The method involves preparing a standard sodium hydroxide solution, standardizing the solution, and titrating the skimmed milk powder solution to determine the protein content.
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